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KMID : 0352519850220010355
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Korea Univercity Medical Journal
1985 Volume.22 No. 1 p.355 ~ p.363
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An Immunohistochemical Study of Carcinoembyonic Antigen, Secretory IgA and Sceretory component in Normal and Carcinomatous Colonic Mucosa
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柳建一/Yoo, Kun Il
崔宗相/金世民/Choi, Jong Sang/Kim, Sae Min
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Abstract
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The predominant immunoglobulin in external secretions of humans and various other mammals is a special form of IgA known as secretory IgA, a molecule composed of a dimer of serum-type IgA plus a J chain and a distinctive polypeptide chain known as secretory component (SC). Histological localization of SC and a chain of IgA in normal mucosa has been studied by ´,immunohistological methods and identified in the cytoplasm of epithelial cells in mucous membranes, spaces between these cells, and secretion. On the other hand, carcinoembryonic antigen is a fetal glycoprotein which may reappear during malignant transformation of entodermal epithelial cell.
The purpose of this study is to compare distribution of SC in normal. and malignant epithelial cell to understand local immunological defense mechanism, especially along the luminal surface replaced by tumor mass.
Each 5 cases of formalin fixed and paraffin embedded well, moderately and poorly ,differentiated adenocarcinoma of colon were selected and immunoperoxidase staining of Avidin-Biotin-Complex method were performed using anti-CEA, SC, and IgA antiserum and ,compared distribution of each antigen between normal mucosa and carcinoma.
The result obtained are as follows :
1. Carcinoma of the colon revealed strong CEA positive reaction along the luminal surface of the gland structure or in the cytoplasm while normal Lieberkuhn epithelial cells showed negative reaction.
2. Normal Lieberkuhn epithelial cells showed SC positive reaction in the cytoplasm while carcinoma cells of the colon disclosed no SC in the cytoplasm, regardless of degree of differentiation of the tumor cell.
3. IgA positive plasma cells were noted mainly along the lamina propria and epithelial cells lining Lieberkuhn´s crypt also revealed positive reaction neither tumo cell nor interstitium revealed any evidence of IgA.
Above findings suggests that mucosa of colon which is replaced by carcinoma is devoid of local immunological defense mechanism as far as SC and IgA are concerned. And disappearance of SC in colonic ca cinoma cell seems to be a useful indicator ill the field of histologic differential diagnosis of metastatic carcinoma.
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KEYWORD
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